Natalie Kuldell

On the chalkboard, Natalie rapidly outlined the design principles we would be exploring to create the living photographs. First she drew an electrical circuit. She explained that these design principles were a simple, electronics-derived way of depicting the series of biochemical reactions that would occur within the living photograph.  Essentially, we would introduce a light sensitive gene (from photosynthetic blue-green algae) into a strain of  E.coli that cause the bacteria to create a black pigment. Red light would activate the mechanism, and bacteria in areas of the dark areas of the photo would digest a certain kind of sugar (S-gal®) that would cause a blackish color to be produced from the bacteria.

Diagram of the bacterial photography process

Now it was time for the hands-on portion of the class. To further support the biology-as-electronics analogy, the class used a breadboard and LEDs to show how the bio chemical reactions would create the circuits. Natalie mused, “If only biology were are predictable as electronics.”

MIT students creating a simulation of the biological circuitry using breadboards.

After everyone had tested their breadboards, it was time to create the actual bacterial photographs. Using pipettes we followed the protocols that had been outlined to create the photoreceptive E.coli. Each Petri dish’s bacteria was suspended in liquid agar to assure adequate distribution, creating a lawn for the bacteria to grow evenly.

A little about the development of these protocols: photosynthetic bacteria created from a dual biological circuit system designed by Dr. Chris Voigt, then an assistant chemistry professor at the University of California, San Francisco (UCSF), now also at MIT. The idea further developed by a group of students at the University of Texas at Austin, as part of the iGEM (intercollegiate Genetically Engineered Machine) competition in 2005 at MIT. (http://2006.igem.org/wiki/index.php/University_of_Texas_2006)

Pouring the agar into the Petri dish

Petri dishes in hand, we selected images to be printed on transparencies. This creates a shaded area that allows the bacteria beneath to activate and produce the sugar-digesting enzyme (beta-galactosidase) that creates the darks in the Petri dish. The UT students explained it nicely (http://www.utexas.edu/features/2005/bacteria/index.html), “When the bacteria grow in dark parts of the Petri dish, they digest the sugar and produce black pigment. Those in the light don’t produce the sugar-digesting enzyme and the film remains clear.”

Students selected a penguin, the Taj Mahal, even a petite handlebar mustache. Wythe selected an octopus rendering from the legendary botanist and artist Ernest Haeckel, and I pulled a dotted unicorn that I had created on my computer. After all images had been loaded onto the thumb drive, Wythe and I accompanied Natalie to the lab where her bacterial darkroom was located.

My unicorn and Wythe’s octopus transparencies taped on top of our Petri dishes

Natalie experimented many years to create the perfect “darkroom” for the bacterial cultures. Identifying the combination of the perfect distance and light intensity was no speedy feat. Her darkroom is constructed in a refrigerator outfitted with a red light and shelving for the Petri dishes to sit on. The outside of the refrigerator is covered with post cards from distant places; monuments to great achievements in beauty and construction from both humans and nature. The gothic Duomo di Milano, the majestic Grand Canyon, a gorgeous selection of Central and South American coastlines… even a Texan cowboy, perhaps a nod to the UT students. Our octopus and unicorn petri dishes safely tucked into the bacterial darkroom, we thanked Natalie and bid her farewell.

The bacterial photography darkroom

Simultaneously exhausted and enlivened, Wythe and I boarded the bus back to New York, chatting the entire journey back about the wonders of biotechnology.

Two days later we received an email from Natalie with several photos of bacterial photography from the class. There, imprinted in the agar were the students’ selections; the Taj Mahal, the penguin, the mustache. Sadly though, we were not bacteria-whispers. Our little microbes precipitated neither fish nor fauna… no octopus, no unicorn. So what had happened? Somewhere we made an error; a fine measurement in the protocols was off—perhaps we had too much of one thing and not enough of another. Who knows? We’ll get it next time!

Successful bacterial photographs created by MIT students with original transparency images

Resources:

About bacterial photography:
http://www.utexas.edu/features/2005/bacteria/index.html
http://2006.igem.org/wiki/index.php/University_of_Texas_2006

About Natalie Kuldell:
http://web.mit.edu/be/people/kuldell.shtml
http://www.biobuilder.org/

Special thanks to Daniel Gruskin for arranging the visit, and Oliver Medvedik for his suggestions on the diagram.

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Images courtesy Daniel Grushkin.

“…the slime mold is a quite intelligent, fascinating being, and even an able collaborator in art…”

Unicorn mosaic made from genetically engineered fluorescent K12 bacteria, crowdsourced from participants at SXSW Create, 2013. Thanks to all participants!

Presenting… the Unicorn Mosaic made from genetically engineered fluorescent K12 bacteria, crowdsourced from participants at SXSW Create, 2013. If you were a contributor, check below for the number that corresponds with the card you received at SXSW to see how your bacteria dish developed.

Thanks to all who participated! You have the distinction of having helped create what Adam Balkan of  New York 1 dubbed: “…probably the world’s first ever giant unicorn-shaped piece of bacteria…” and it came out great!

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(200 Photos)

For centuries, artists and designers have looked to nature for inspiration and for materials, but only recently have they become able to incorporate living organisms or tissues into their work. This startling development at the intersection of biology and design has created new aesthetic possibilities and can help address the growing urgency to build and manufacture ecologically.

In this talk, William Myers, author of the new book BioDesign: Nature + Science + Creativity, will present several recent experiments in harnessing biology for art and design: from thoroughly serious and practical applications to provocative, gorgeous works of art. Highlights include a portrait of the human microbiome, a footbridge supported by willow trees, packaging made with mushrooms and a scheme to use bacteria to solidify sand dunes into walls in the desert.

A Q&A and book signing will follow the presentation.

This event is part of CUT/PASTE/GROW.

Stay tuned- we’ll be sharing updates as we continue to create… The World’s First Ever Giant Unicorn-shaped Piece of Bacteria!

The CREATE tent at SXSW, pre-set up. This was where the magic happened, y’all!

Daniel Grushkin of Genspace explains the engineering behind the phosphorescence in the bacteria, as well as how to use the bacteria as “paint” for the mosaic to an anticipatory participant and an onlooker.

Golgi apparatus or abstract expressionism?

Virginia Ingram traces over her design in a petri dish with bacteria that will glow green.

CUT/PASTE/GROW mosaic artists at work.

The finished tiles. Next up for them, a little “culture”!

Andrew & Andrew are down with the bioart!

Help Genspace, Observatory, and the Empiricist League create a crowd-sourced living, glowing mosaic of bacterial paintings. Each mosaic tile will be painted on a petri dish with K-12 bacteria. The DNA of this bacteria has been genetically modified to glow green or red under a black light. All the tiles together will create the shape of the ultimate genetic hybrid, the unicorn. The mosaic will be part of an art exhibit featuring a dozen of today’s most dynamic bioartists in Brooklyn, NY.

Each participant will receive a souvenir postcard on which they’ll be able to draw the image that will appear on their mosaic tile. Participants will then trace over their drawing in a petri dish with their bacterial paint brush. After a few days, when the bacterial colonies have grown, the tiles will be assembled under a black light and photographed in a unicorn formation. The photograph will be posted on cutpastegrow.com where participants will be able to view their contributions to the mosaic.